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Interleukin 3, Recombinant, Human (IL-3)
Interleukin 3, Recombinant, Human (IL-3)
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| Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15.0kDa protein containing 133 amino acid residues. | | | Catalog # | I7663-35 | | Biological Activity | Human IL-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0x10e7 units/mg. | | Responding Cells (partial list) | Certain BM precursor cells | | Concentration Range for most in vitro applications | 0.1-10ng/ml. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | | Country of Origin | USA | | Molecular Weight | 15.0kD | | Source | Recombinant, Human (E. coli). | | Purity | 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug). | | Form | Supplied as a lyophilized protein with no additives. | | Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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