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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Human (IL-3)

Interleukin 3, Recombinant, Human (IL-3)

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Specifications

Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15.0kDa protein containing 133 amino acid residues.
Catalog #I7663-35
Biological Activity Human IL-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0x10e7 units/mg.
Responding Cells (partial list) Certain BM precursor cells
Concentration Range for most in vitro applications 0.1-10ng/ml.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Country of OriginUSA
Molecular Weight15.0kD
SourceRecombinant, Human (E. coli).
Purity98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug).
FormSupplied as a lyophilized protein with no additives.
Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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