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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Human (IL-3)

Interleukin 3, Recombinant, Human (IL-3)

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Specifications

Recombinant Human IL-3 is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton.
Catalog #I7663-35A
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityrHuIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of rHuIL-3.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
ReconstitutionReconstitute the lyophilized rHuIL-3 in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in HSA, sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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