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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Human (IL-3)

Interleukin 3, Recombinant, Human (IL-3)

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Specifications

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases
Catalog #I7663-35C
DescriptionRecombinant Human IL-3 produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. Recombinant IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Pro-Thr.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRecombinant IL-3 Sf9 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Human IL-3.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
GeneName: IL3
SolubilityIt is recommended to reconstitute the lyophilized IL-3 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Recombinant Human IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant IL-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight15.0kD
SourceSf9 insect cells
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 0.5X PBS, pH 7.4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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