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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Mouse (IL-3)

Interleukin 3, Recombinant, Mouse (IL-3)

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Specifications

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1kD globular protein containing 135 amino acid residues.
Catalog #I7663-36
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is
0.05ng/ml, corresponding to a specific activity of 2 x 10e7u/mg.
AA SequenceMDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Molecular Weight15.1kD
SourceE. coli.
PurityHighly Purified ( 98% by SDS-PAGE analysis).
Endotoxin0.1ng/ug (1EU/ug).
FormSupplied as a lyophilized protein without additives. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml with carrier protein (0.1% HSA or BSA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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