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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Mouse (IL-3)

Interleukin 3, Recombinant, Mouse (IL-3)

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Specifications

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases
Catalog #I7663-36A
DescriptionRecombinant murine IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. Recombinant Murine IL-3 is purified by proprietary chromatographic techniques.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityMurine IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 2 x 107 IU/mg.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Murine IL-3 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight15.1kD
SourceE. coli
Purity97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified .Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives. Reconstitute in sterile ddH2O to 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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