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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Human (IL-3, Burst Promoting Activity, Colony Stimulating Factor Multiple, Eosinophil Colony Stimulating Factor, Hematopoietic Growth Factor, Mast Cell Growth Factor, MCGF, MGC79398, MGC79399, Multilineage Colony Stimulating Fa

Interleukin 3, Recombinant, Human (IL-3, Burst Promoting Activity, Colony Stimulating
Factor Multiple, Eosinophil Colony Stimulating Factor, Hematopoietic Growth Factor, Mast
Cell Growth Factor, MCGF, MGC79398, MGC79399, Multilineage Colony Stimulating Fa

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Specifications

Interleukin-3 Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15.0 kD The IL-3 is purified by proprietary chromatographic techniques.
Catalog #I7663-41A
SourceProtein factor IL-3 human recombinant expressed in E. coli. Amino Acid Sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard.
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 10e7 IU/mg.
Storage and StabilityFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Molecular Weight15.0kD
SourceE. coli
Purity98.0% as determined by RP-HPLC or SDS-PAGE.
ConcentrationAs reported
FormSupplied lyophilized from a concentrated (1mg/ml) solution in water containing 5mg HSA and 4.3mg Sodium Chloride. Reconstitute in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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