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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 3, Recombinant, Human (Interleukin-3, IL3, IL-3, Hematopoietic Growth Factor, Mast Cell Growth Factor, MCGF, MGC79398, MGC79399, Multipotential Colony-stimulating Factor, MULTI-CSF, P-cell-stimulating Factor)

Interleukin 3, Recombinant, Human (Interleukin-3, IL3, IL-3, Hematopoietic Growth Factor,
Mast Cell Growth Factor, MCGF, MGC79398, MGC79399, Multipotential Colony-stimulating
Factor, MULTI-CSF, P-cell-stimulating Factor)

Pricing

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Specifications

Catalog #I7663-41F
SourceRecombinant corresponding to 133aa of human Interleukin-3 expressed in E.coli is a single, non-glycosylated polypeptide chain.
Molecular Weight~15kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1ng/ml, corresponding to a Specific Activity of 1x10 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~15kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesInterleukin 3 (Colony-stimulating Factor, Multiple)


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