USBio Logo

I7663-97B Goat Anti-Interleukin 33 (IL-33)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Mouse
Swiss Prot
Q8BVZ5
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IC IHC N WB
Crossreactivity
Mo
Gene ID
77125
Shipping Temp
Blue Ice
Storage Temp
-20°C

IL-33 is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells.

Applications
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, ELISA, Immunohistochemistry and Neutralization. Other applications not tested.
Recommended Dilutions
Western Blot: 0.4ug/ml using HEK293T human embryonic kidney cell line transfected with full length mouse IL-33. Flow Cytometry (Intracellular): 2.5ug/10e6 mouse splenocytes treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 5-15ug/ml using Immersion fixed bEnd.3 mouse endothelioma cell line Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse spleen ELISA (Capture): 0.2-0.8ug/ml Neutralization: 10-50ng/ml in the presence of 0.25ng/ml of recombinant mouse IL-33. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser109-Ile266 from mouse IL-33 expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by Immunoaffinity chromatography.
Specificity
Recognizes mouse IL-33. In sandwich immunoassays, less than 2% crossreactivity is observed with recombinant human IL-33.
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved