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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 33, Recombinant, Human (IL-33)

Interleukin 33, Recombinant, Human (IL-33)

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Specifications

IL-33 is a member of the IL-1cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells. Recombinant human IL-33 is anon-glycosylated protein, consisting of 160aa and having a total molecular weight of 18.1kD.
Catalog #I7663-97E
Recombinant protein corresponding to human IL-33, expressed in E. coli
Biological ActivityThe activity is determined by the ability to induce the proliferation of D10.G4.1 cells co-stimulated with anti-CD3 and is typically 0.06-0.24ng/ml.
Amino Acid SequenceMSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.1kD
SourceE. coli
Purity95% as determined by reducing and non-reducing SDS-PAGE
FormSupplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Endotoxin: 0.1EU/ug (LAL)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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