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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)

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Specifications

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta. In pheochromocytoma cells, NGF induces the synthesis of IL1.
Catalog #I7663A
Recombinant Human IL-1 is a single, non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Phe-Ser.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityrHuIL-1alpha is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is less then 0.001ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug)
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard.
ReconstitutionReconstitute the lyophilized rHuIL-1alpha in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in aliquots at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in 20mM Tris-HCl, pH 8.0, 5mM MgCl2, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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