Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)
Pricing
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| A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta. In pheochromocytoma cells, NGF induces the synthesis of IL1. | | | Catalog # | I7663A | | Recombinant Human IL-1 is a single, non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Phe-Ser. | | Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | rHuIL-1alpha is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is less then 0.001ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg. | | Endotoxin | 0.1ng/ug (IEU/ug) | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard. | | Reconstitution | Reconstitute the lyophilized rHuIL-1alpha in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. | | Storage and Stability | Lyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in aliquots at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder in 20mM Tris-HCl, pH 8.0, 5mM MgCl2, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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