Interleukin 1 alpha, Recombinant, Porcine (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)
Pricing
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| A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta . In pheochromocytoma cells, NGF induces the synthesis of IL1. | | | Catalog # | I7663Q | | Description | Recombinant Porice IL-1 alpha produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. Porcine IL-1a is purified by proprietary chromatographic techniques. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | IL-1a is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of D10S cells is < 0.03 ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of IL-1alpha. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of IL-1a as a Reference Standard. | | Gene | Name: IL1A | | Solubility | It is recommended to reconstitute the lyophilized Porcine IL-1 alpha in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | | Stability | Lyophilized Porcine IL-1a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Porcine IL-1alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 18.08kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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