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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 1 alpha, Recombinant, Porcine (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

Interleukin 1 alpha, Recombinant, Porcine (IL-1a, Hematopoietin-1, Lymphocyte-activating
Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell
Factor, MCF)

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Specifications

A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta . In pheochromocytoma cells, NGF induces the synthesis of IL1.
Catalog #I7663Q
DescriptionRecombinant Porice IL-1 alpha produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. Porcine IL-1a is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityIL-1a is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of D10S cells is < 0.03 ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of IL-1alpha.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1a as a Reference Standard.
GeneName: IL1A
SolubilityIt is recommended to reconstitute the lyophilized Porcine IL-1 alpha in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Porcine IL-1a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Porcine IL-1alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight18.08kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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