Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 4, Recombinant, Mouse (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pit

Interleukin 4, Recombinant, Mouse (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin,
HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1,
Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pit

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant murine IL-4 is a 13.5kD globular protein containing 120 amino acid residues.
Catalog #I8426-10
AA Sequence MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Biological Activity Fully biologically active when compared to standards. The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is < 2ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
Responding Cells (partial list) B cells and certain T cells
Concentration Range for most in vitro applications 0.5-5ng/ml
Storage and StabilityLyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in working aliquots at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight13.5kD
SourceMurine, recombinant protein expressed in E. coli
Purity98% by SDS-PAGE analyses.
Endotoxin0.1ng/ug (1EU/ug).
FormSupplied as a lophilized protein without additives.
ReconstitutionReconstitute in water to a concentration of 1mg/ml. This solution can then be diluted into other aqueous buffers.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links