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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 4, 25-153 aa, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MG

Interleukin 4, 25-153 aa, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF1,
Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory
Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MG

Pricing

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Specifications

Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naïve helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Catalog #I8426-16E
AA SequenceMGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT
AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Endotoxin Level 1.0 EU per 1 ug of protein (determined by LAL method)
Biological activityThe ED50 for this effect is <1.8ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.2kD
SourceE. coli
Purity95% by SDS-PAGE
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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