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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 4, Recombinant, Rat (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitra

Interleukin 4, Recombinant, Rat (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin,
HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1,
Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitra

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Specifications

Interleukin-4 (IL4) has a compact, globular fold (similar to other cytokines), stabilized by 3 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet.
Catalog #I8426-26B
Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumor cells. IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.
Recombinant Rat IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105D.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys Asn.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRecombinant Rat IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent prolifiration of Con A activated rat spleen cells is 1.5ng/ml, corresponding to a Specific Activity of 6.6 x 10e5 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of Interleukin-4
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.
GeneName: Il4; Synonyms: Il-4
SolubilityIt is recommended to reconstitute the lyophilized IL-4 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
StabilityRecombinant Rat IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Interleukin-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight14.11kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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