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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 4, Recombinant, Mouse (Interleukin-4, IL-4, IL4, B-cell Stimulatory Factor 1, BSF-1, Lymphocyte Stimulatory Factor 1)

Interleukin 4, Recombinant, Mouse (Interleukin-4, IL-4, IL4, B-cell Stimulatory Factor 1,
BSF-1, Lymphocyte Stimulatory Factor 1)

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Specifications

IL-4 is produced by T cells, NK T cells, gamma delta cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces the polarization of naïve helper T cells into the TH2 phenotype (1,2). IL-4 also promotes B cell proliferation, antibody class switching and the production other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (3). IL-4 binds to two distinct receptors, the Type I receptor and Type II receptor. Type I receptor is a heterodimer consisting of IL-4Ra chain and the common gamma chain, gc (4,5). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Ra and IL-13Ra1. Signaling initiated via Type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (4). The Type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (4).
Catalog #I8426-33Q
SourceRecombinant corresponding to aa21-140 of mouse IL-4 expressed in human 293 cells (NP_067258).
BioactivityDetermined in an HT-2 cell proliferation assay. The ED50 is 100-400pg/ml.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity~98% (SDS-PAGE, reduced and non-reduced). Endotoxin: 0.01ng/1ug.
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.22um filtered solution of PBS, pH 7.2, 20ug
BSA/1ug mIL-4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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