Interleukin 4, Recombinant, Porcine (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin,
HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1,
Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, P
Pricing
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| Interleukin-4 (IL4) has a compact, globular fold (similar to other cytokines), stabilized by 3 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. | | | Catalog # | I8426-42D | | Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumor cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes. Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses. | | Recombinant Porcine IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615D. Recombinant Porcine IL-4 is purified by proprietary chromatographic techniques. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Porcine IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is < 10 ng/ml, corresponding to a Specific Activity of 1 x 10e5 IU/mg. | | Endotoxin | 0.1ng/ug (IEU/ug) of Porcine IL-4. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. | | Gene | Name: IL4 | | Solubility | It is recommended to reconstitute the lyophilized Porcine IL-4 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. | | Stability | Recombinant Porcine IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 12.62kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Interleukin 4, Recombinant, Porcine (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, P |
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