Interleukin 4, Recombinant, Rabbit (Interleukin-4, IL-4, B-cell Stimulatory Factor 1,
BSF-1, Lymphocyte Stimulatory Factor 1, IL4)
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| Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, ~13kD-18kD Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four a-helix structure. Rabbit IL-4 is synthesized with a 24aa signal sequence. Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Ra and the common G chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 Ra and IL-13 Ra1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. | | | Catalog # | I8426-43F | | Source | Recombinant corresponding to aa25-147 from rabbit IL-4, fused to N-terminal Met, expressed in E.coli. | | Molecular Weight | ~14.2kD | | Biological Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is typically 3-15ng/ml. | | Endotoxin | ~1EU/1ug (LAL) | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~14.2kD | | Source | E. coli | | Purity | ~95% (SDS-PAGE) | | Concentration | ~0.1mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, BSA. Reconstitute with PBS, 0.1% human or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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