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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 5, Recombinant, Human (Interleukin-5, IL5, IL-5, B Cell Differentiation Factor I, Eosinophil Differentiation Factor, EDF, T-cell Replacing Factor, TRF)

Interleukin 5, Recombinant, Human (Interleukin-5, IL5, IL-5, B Cell Differentiation Factor
I, Eosinophil Differentiation Factor, EDF, T-cell Replacing Factor, TRF)

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Specifications

Catalog #I8427-01D
SourceRecombinant corresponding to 132aa of human Interleukin-5 expressed in E.coli is a dimeric, non-glycosylated polypeptide chain.
Molecular Weight~26.5kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells was foundto be less then 0.15ng/ml, corresponding to a Specific Activity of 6x10 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.625 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-5 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~26.5kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesInterleukin 5 (Colony-stimulating Factor, Eosinophil)


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