Interleukin 5, Recombinant, Human (Interleukin-5, IL5, IL-5, B-cell Differentiation Factor
I, Eosinophil Differentiation Factor, EDF, T-cell Replacing Factor, TRF)
Pricing
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| Catalog # | I8427-01D | | Source | Recombinant corresponding to 132aa of human Interleukin-5 expressed in E.coli is a dimeric, non-glycosylated polypeptide chain. | | Molecular Weight | ~26.5kD | | Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells was foundto be less then 0.15ng/ml, corresponding to a Specific Activity of 6x10 IU/mg. | | Dimmers and Aggregates | 1% (SDS-PAGE) | | Protein Content | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.625 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-5 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~26.5kD | | Purity | ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Interleukin 5 (Colony-stimulating Factor, Eosinophil) |
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