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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor, HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon beta

Anti -Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell
Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor,
HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon
beta

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IH RIA N
IL-6 is a cytokine with a wide variety of biological functions. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
Catalog #I8428-02L
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Neutralization and RIA. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:5000
Western Blot: 1:500-1:2000 Detects IL-6 in supernatants and lysates of 2x10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PMBC are stimulated for 24 hours with 1% human serum plus 10ng/ml E. coli LPS. Multiple bands bands will appear due to the variable amounts of glycosylation on the molecule (21-21kD).
Immunoprecipitation: 1:400-1:800; Precleaning with a non-specific rabbit IgG reduces background.
Immunohistochemistry (Frozen, paraffin): 1:400-1:800
Neutralization: 1:400. Incubate with sample for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.
RIA: 1:4000-1:8000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PuritySerum
ImmunogenRecombinant corresponding to human IL-6 expressed in E. coli.
Cellular LocalizationSecreted
SpecificityRecognizes native and recombinant human IL-6. Species Crossreactivity: mouse, porcine and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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