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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 6, Recombinant, Human (IL-6)

Interleukin 6, Recombinant, Human (IL-6)

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Specifications

Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6R and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells. Recombinant human IL-6 is a non-glycosylated protein, containing 184 amino acids and having a molecular weight of 21 kDa.
Catalog #I8428-05C
SequencePVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Biological Activity Recombinant Human IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine 7TD1 cells is 0.16-0.24ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 10mM HCl, aliquot and store at -20°C. It is recommended for long-term storage to add a carrier protein 0.1% HSA or BSA. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight21kD
SourceE. coli
Purity95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: 1EU/ug (LAL)
FormSupplied as a lyophilized powder from 10mM acetic acid. Reconstitute with10mM HCl to a concentration of 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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