Anti -Interleukin 8 (IL-8, 3-10C, AMCF-I, b-ENAP, CXCL8, C-X-C Motif Chemokine 8, Emoctakin, GCP1, GCP-1, Granulocyte Chemotactic Protein 1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, Monocyte-derived Neutrophil-activating Peptide, Monocyte-derived Neutrophil chemot
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH N |
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| IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. | | | Catalog # | I8430-03L | | Applications | Suitable for use in ELISA, Western Blot, Neutralization Studies and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | ELISA: 0.5-2ug/ml; Use at 100ul per well, detects 0.2-0.4ng human IL-8 per well. | | Western Blot: 0.1-0.2ug/ml detects 1.5-3.0ng human IL-8 | | per lane under reducing and non-reducing conditions. | | Neutralization Studies: 2-3ug/ml is found to yield half-maximal inhibition of the biological activity of 100ng/ml human IL-8. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly purified (>98% pure) recombinant protein corresponding to 17aa of human endothelial IL-8. | | Specificity | Recognizes all forms of natural and recombinant human IL-8. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Interleukin 8 (IL-8, 3-10C, AMCF-I, b-ENAP, CXCL8, C-X-C Motif Chemokine 8, Emoctakin, GCP1, GCP-1, Granulocyte Chemotactic Protein 1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, Monocyte-derived Neutrophil-activating Peptide, Monocyte-derived Neutrophil chemot |
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