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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. Human IL-10 is active on murine cells. Murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6kD protein with 161 amino acid residues.
Catalog #I8432-01F
ApplicationSuitable for use in ELISA as a capture antibody. Other applications have not been tested.
Recommended Dilutions
ELISA: 1-5ug/ml; Coat each well with 100ul.
Optimal dilutions to be determined by the researcher.
Recommended PairSuitable for use as the capture or solid phase antibody.
Matched to I8432-16 as the detection or conjugate (label) antibody.
HybridomaBALB/c splenocytes were fused with NSO mouse myeloma cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5J48
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman Interleukin 10
SpecificityRecognizes natural and recombinant human IL-10. Binds to epitope B of the IL-10 protein and is non-neutralizing. Does not bind to the Epstein Barr viral IL-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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