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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH FC
IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells. IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.
Catalog #I8432-02E1
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.
Western Blot: 1:1000
ELISA: 1:200-1:2000
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
HybridomaP3.X63 Ag8.653 myeloma cells with spleen cells from immunized rats.
Recommended Neagtive ControlI1904-76H: IgG1 Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B2354
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant mouse IL-10
SpecificityRecognizes mouse IL-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMGC126450, MGC126451, TGIF


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