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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH FC
Interleukin-10 (IL-10) is produced by a population of T cells. IL-10 is expressed by activated CD8+ peripheral blood T-cells, activated T-helper CD4+ T cell clones mast cells, B cell lymphomas and LPS activated monocytes. It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response. Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Catalog #I8432-02K
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.
Western Blot: 1:100-1:250
Immunohistochemistry (Paraffin): 1:100. Requires antigen retrieval using heat treatment and sodium citrate buffer, pH 6.0, prior to staining.
Optimal dilutions to be determined by the researcher.
IHC Positive ControlHuman tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B2362
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in borate buffered saline, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman IL-10 expressed by COS cells.
SpecificityRecognizes human IL-10. Reported to neutralize the effects of IL-10. I8432-02KX is recommend for such studies.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMGC126450, MGC126451, TGIF


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