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Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
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| Catalog # | I8432-09 | | Description | Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. | | Source | E.coli | | Form | The sterile filtered solution was lyophilized from 5 mM Tris, pH 7.2. | | Stability | The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted IL-10 should be stored in working aliquots at -20°C. | | Purity | 98% by SDS-PAGE and HPLC. | | Endotoxin | 0.1ng/ug (1EU/ug) | | Reconstitution | We recommend a quick spin followed by reconstitution in water to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | | Biological Activity | The ED50 as determined by the dose-dependent inhibition of antigen-specific T cell proliferation is less than 10ng/ml. | | AA Sequence | MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNILTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINYI EAYVTLKMKN | | Country of Origin | USA | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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