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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Catalog #I8432-09
Description Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.
Source E.coli
Form The sterile filtered solution was lyophilized from 5 mM Tris, pH 7.2.
Stability The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted IL-10 should be stored in working aliquots at -20°C.
Purity 98% by SDS-PAGE and HPLC.
Endotoxin 0.1ng/ug (1EU/ug)
Reconstitution We recommend a quick spin followed by reconstitution in water to a concentration of 0.11.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Biological Activity The ED50 as determined by the dose-dependent inhibition of antigen-specific T cell proliferation is less than 10ng/ml.
AA Sequence MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNILTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINYI EAYVTLKMKN
Country of Origin USA
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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