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Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Pricing
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| Catalog # | I8432-09A | | Source | Recombinant corresponding to rat IL-10 expressed in ~E. coli | | Molecular Weight | ~18.7kD | | Activity | ED50 <10ng/ml (>1x10e5units/mg). The biological activity is determined in an assay measuring dose-dependent inhibition of antigen-specific T cell proliferation. For most in vitro applications, IL-10 is effective at a concentration range of 0.2-20ng/ml. The optimal concentration should be determined for each specific application. | | Endotoxin | ~0.1ng/ug | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile Tris. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 18.7kD | | Source | E. coli | | Purity | ~98% (SDS-PAGE, HPLC) | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in 5mM Tris, pH 7.2. Reconstitute with 5mM Tris, pH 7.2, to 0.1-1.0 mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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