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You are here:Home » Molecular Biology » MB-Growth Factors-Interleukin » Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Interleukin 10, Recombinant, Rat (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)


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Catalog #I8432-09A
SourceRecombinant corresponding to rat IL-10 expressed in ~E. coli
Molecular Weight~18.7kD
ActivityED50 <10ng/ml (>1x10e5units/mg). The biological activity is determined in an assay measuring dose-dependent inhibition of antigen-specific T cell proliferation. For most in vitro applications, IL-10 is effective at a concentration range of 0.2-20ng/ml. The optimal concentration should be determined for each specific application.
Endotoxin ~0.1ng/ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile Tris. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.7kD
SourceE. coli
Purity~98% (SDS-PAGE, HPLC)
ConcentrationNot determined
FormSupplied as a lyophilized powder in 5mM Tris, pH 7.2. Reconstitute with 5mM Tris, pH 7.2, to 0.1-1.0 mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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