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Interleukin 10, Recombinant, Porcine (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Interleukin 10, Recombinant, Porcine (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
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| Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11 . Murine keratinocytes also produce IL10. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0 , Th1 , and Th2 ) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium . The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10. | | | Catalog # | I8432-10R | | Description | Recombinant Porcine IL-10 produced in E.coli is a single, glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18458 Dalton. Procine IL-10 is purified by proprietary chromatographic techniques. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Lys-Ser. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Procine IL-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of MC/9 cell which was found to be < 15ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of Procine IL-10. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.36 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard. | | Gene | Name: IL10 | | Solubility | It is recommended to reconstitute the lyophilized IL-10 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | | Stability | Lyophilized Procine IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-10 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 18.46kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | As reported | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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