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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor
(CSIF), shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. Human IL-10 is active on murine cells. Murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6kD protein with 161 amino acid residues.
Catalog #I8432-11
AA SequenceMSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Biological ActivityDetermined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5x10e5 units/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
SourceHuman, recombinant expressed in E. coli
Purity98% by SDS-PAGE and HPLC
Endotoxin0.1ng/ug
FormSupplied as a lyophilized powder from 5mM sodium phosphate, pH 7.2, without additives. Reconstitute with 5mM Tris, pH 7.2 to a concentration of 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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