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Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
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| Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium. The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10. | | | Catalog # | I8432-11A | | Recombinant Human IL-10 is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.6kD. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. | | Biological Activity | Interleukin-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 10e7 IU/mg. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 18.6kD | | Source | Recombinant, Human (CHO cells) | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: Less than 0.1ng/ug (IEU/ug) . | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder in 60mM PBS, pH 6.5, 100mM sodium chloride. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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