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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium. The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10.
Catalog #I8432-11A
Recombinant Human IL-10 is a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.6kD.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
Biological ActivityInterleukin-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 10e7 IU/mg.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight18.6kD
SourceRecombinant, Human (CHO cells)
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: Less than 0.1ng/ug (IEU/ug) .
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in 60mM PBS, pH 6.5, 100mM sodium chloride. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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