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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B N

Interleukin-10 is produced by a population of T cells. It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response. Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Catalog #	I8432-13F
Applications	Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended Dilution	ELISA: 0.5ug/ml
	Western Blot: 0.1-0.2ug/ml
	Neutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50).
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents	Sheep anti Rabbit IgG: HRP
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant mouse IL-10.
Specificity	Recognizes mouse interleukin-10 (IL-10).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.