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You are here:Home » Antibodies » Antibodies-Interleukin » Anti -Interleukin 10 (IL-10)

Anti -Interleukin 10 (IL-10)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Interleukin-10 is produced by a population of T cells. It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response. Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Catalog #I8432-13F
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionELISA: 0.5ug/ml
Western Blot: 0.1-0.2ug/ml
Neutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50).
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsSheep anti Rabbit IgG: HRP
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant mouse IL-10.
SpecificityRecognizes mouse interleukin-10 (IL-10).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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