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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10)

Anti -Interleukin 10 (IL-10)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Interleukin-10 is produced by a population of T cells. It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response. Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Catalog #I8432-13F
ApplicationsSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.
Recommended DilutionELISA: 0.5ug/ml
Western Blot: 0.1-0.2ug/ml
Neutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50).
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsSheep anti Rabbit IgG: HRP
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant mouse IL-10.
SpecificityRecognizes mouse interleukin-10 (IL-10).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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