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Specifications

Clone Host Grade Applications Monoclonal Rat Purified E B FC N

Human Interleukin-10 (IL-10), an 18kD cytokine, is secreted by Th2 and B-1 cells.
Catalog #	I8432-13H
Applications	Used for capture of human IL-10 by ELISA, intracellular staining and flow cytometric analysis, Western blotting, and for neutralizing IL-10 binding and biological activity. Tested by sandwich ELISA for detection of human IL-10. It is recommended that the antibody be carefully titrated for optimal performance in the assay conditions used.
Recommended Dilution	ELISA: 2ug/ml
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	4H183
Host	Rat
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 150mM sodium chloride, 0.09% sodium azide.
Purity	Purified
Specificity	Recognizes human Interleukin 10 (IL-10)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.