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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B FC N
Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response and stimulation of the Th2 cell response which results in elevated antibody production.
Catalog #I8432-13K
ApplicationsSuitable for use in Flow Cytometry, ELISA, Neutralization and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5ug/1x10e6 cells
ELISA: 2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlRecombinant IL-10 or activated monocytes
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No11E237
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to IL-10 expressed in Cos cells.
SpecificityRecognizes human IL-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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