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Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B N |
|
| Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. | | | Catalog # | I8432-14 | | Applications | Suitable for use in Neutralization, Sandwich ELISA and Western Blot. Other applications have not been tested. | | Recommended Dilutions | Sandwich ELISA: 0.5-2ug/ml to detect rat IL-10 using 100ul/well antibody solution. Used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant rat IL-10. | | Western Blot: 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Country of Origin | USA | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. | | Purity | Purified by affinity chromatography. | | Immunogen | Highly purified (>98%), recombinant rat Interleukin-10, expressed in E. coli | | Specificity | Recognizes rat Interleukin-10. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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