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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production.
Catalog #I8432-14
ApplicationsSuitable for use in Neutralization, Sandwich ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionsSandwich ELISA: 0.5-2ug/ml to detect rat IL-10 using 100ul/well antibody solution. Used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant rat IL-10.
Western Blot: 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Country of Origin USA
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by affinity chromatography.
ImmunogenHighly purified (>98%), recombinant rat Interleukin-10, expressed in E. coli
SpecificityRecognizes rat Interleukin-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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