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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B
Catalog #I8432-18A
ApplicationsELISA Capture/Detection: Useful as a capture or a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels.1-5 Purified antibody can be paired with biotinylated antibody (Cat. No. I8432-24C) as the detection antibody, with purified recombinant mouse IL-10 as the standard.
Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100ng/lane of recombinant mouse IL-5 under reducing conditions.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K315
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenE. coli-expressed recombinant mouse IL-10
SpecificityRecognizes mouse Interleukin-10 (IL-10).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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