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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B FC IF
Catalog #I8432-25A
ApplicationsELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified antibody should be paired with biotinylated monoclonal antibody as the detection antibody, with purified recombinant human IL-10 as the standard.
Immunofluorescence/Flow Cytometry: The antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control.
Western Blotting: The purified antibody is also useful for Western blotting applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-10 under reducing conditions.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K303
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenCOS-expressed human IL-10
SpecificityRecognizes human and viral Interleukin-10 (IL-10).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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