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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10) (PE)

Anti -Interleukin 10 (IL-10) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified FC
Catalog #I8432-26
ApplicationsSuitable for use in Flow cytometry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application. We recommend the use of Leucoperm for this purpose
Optimal working dilutions to be determined by researcher.
HybridomaSpleen cells from immunized rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Storage and StabilityLyophilized powder may be stored at 4°C before opening. DO NOT FREEZE! Reconstitute to nominal volume by adding ddH2O. Aliquot and store at 4ºC. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2553
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide, 0.5% BSA. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
PurityPurified
ImmunogenRecombinant Mouse IL-10.
SpecificityRecognizes natural and recombinant mouse Interleukin-10. This conjugate is specifically designed for the detection of intracellular IL-10 using flow cytometry.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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