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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Anti -Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH N
Catalog #I8432-27
ApplicationsSuitable for use in ELISA, Western Blot, Neutralization and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000. Natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.
Immunohistochemistry: 1:1000-1:15000
Neutralization: 1:400. Incubation Time: >4 hours.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.01% sodium azide. No stabilizing proteins added.
PuritySerum
ImmunogenHuman IL-10
SpecificityRecognizes recombinant and native human IL-10 present in body fluids and cell supernatants. Will not neutralize the biological activity of murine IL-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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