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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 11, Recombinant, Human (Interleukin-11, IL-11, IL11, Adipogenesis Inhibitory Factor, AGIF, Oprelvekin)

Interleukin 11, Recombinant, Human (Interleukin-11, IL-11, IL11, Adipogenesis Inhibitory
Factor, AGIF, Oprelvekin)

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Specifications

Catalog #I8433-03J
SourceRecombinant corresponding to 179aa of human Interleukin-11 expressed in E.coli is a single, non-glycosylated polypeptide chain.
Molecular Weight~19.3kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of mouse 7TD1 was found to be less then 10ng/ml, corresponding to a Specific Activity of 1x10 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.95 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-11 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~19.3kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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