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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 12, Recombinant, Mouse (IL-12)

Interleukin 12, Recombinant, Mouse (IL-12)

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Specifications

IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Recombinant murine IL-12 is a 75.0kD heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40kD (p40) subunits (506 total amino acid residues).
Catalog #I8434
Biological ActivityED50 as determined by the stimulation of IFN-g production by murine splenocytes co-stimulated with IL-18 is <0.1ng/ml, corresponding to a specific activity of 1x10e7.
Amino Acid Sequencep35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SS p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight75kD
SourceCHO cells
Purity95% by SDS-PAGE and HPLC analysis. Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.1-1mg/ml. Do not vortex. Add protein carrier for long term storage.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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