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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 12, Recombinant, Rat (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)

Interleukin 12, Recombinant, Rat (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL
Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T
cell Stimulating Factor, TSF)

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Specifications

Interleukin 12 (IL-12) is a cytokine with important regulatory functions bridging innate and adaptive immunity. It has been proposed as an immune adjuvant for vaccination therapy of infectious diseases and malignancies. The inflammatory properties of IL-12 play an important role in the adjuvant. Interleukin 12 (IL-12) is a proinflammatory cytokine composed by two chains, p40 and p35.It plays a key role in the promotion of a Th1 immune response in the gastrointestinal mucosa. An enhanced expression of IL-12 mRNA in gastric mucosa has been reported in individuals infected by Helicobacter pylori.
Catalog #I8434-17A
Recombinant Rat IL-12 is a glycosylated disulfide linked heterodimeric polypeptide containing 503aa and having a molecular weight of 70kD comprised of disulfide-bonded 35kD (p35) and 40kD (p40) subunits.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Biological ActivityED50 range=0.01-0.05ng/ml as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated splenocytes that have been costimulated with PMA.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight70kD
SourceRecombinant corresponding to 503aa of Rat IL-12 expressed in Sf9 insect cells.
Purity95% (SDS-PAGE).
FormSupplied as a lyophilized powder without additives. Reconstitute with sterile dH2O to >0.1mg/ml. For long term storage it is recommended to add 0.1% HSA or BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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