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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 12 p35, Recombinant, Human

Interleukin 12 p35, Recombinant, Human

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Specifications

Recombinant Interleukin-12p35 comprises a 197 amino acid fragment (57-253) corresponding to the mature Interleukin-12p35 chain protein and is expressed in E. coli with an amino-terminal hexahistidine tag.
Catalog #I8434-29G
Active IL-12 is a p70 disulfide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman, Recombinant, E. coli
PurityPurified
FormSupplied as a liquid in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1% SDS, 0.1% bromophenol blue, 2.5% glycerol).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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