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Specifications

Catalog #	I8434-29H
Source	Recombinant corresponding to aa57-253 from the mature human Interleukin 12 p35 chain protein with an amino-terminal hexahistidine tag expressed in E. coli.
	Active IL-12 is a p70 disulfide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
Applications	Suitable for use in ELISA. Other applications not tested.
	Recommended Dilution:
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	27.04kD
Source	E. coli
Purity	Purified. 95% by SDS-PAGE.
Concentration	As reported
Form	Supplied as a liquid.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.