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You are here:Home » Molecular Biology » Growth Factors-Interleukin » Interleukin 13, Recombinant, Human (IL-13)

Interleukin 13, Recombinant, Human (IL-13)

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Interleukin-13 (IL-13) is a multifunctional cytokine whose net principle action is to diminish inflammatory responses. Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by human B cells. Previous studies showed that polymorphisms in the IL-13 gene were associated with serum total IgE or allergic asthma. Interleukin (IL)-13 is a pleiotropic cytokine, which shares many biological functions with IL-4. The receptor subunits of IL-13 consist of IL-4Ralpha, IL-13Ralpha1 and IL-13Ralpha2.
Catalog #I8435-10B
DescriptionRecombinant Human IL-13 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12.5kD. Recombinant IL-13 is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and found to be Ser-Pro-Gly-Pro-Val.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityHuman IL-13 is fully biologically active when compared to standard. The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be < 1ng/ml, corresponding to a specific activity of >1 x 106.
Endotoxin 0.1ng/ug (IEU/ug) of recombinant Human IL-13.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized Human IL-13 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Recombinant Human Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Human Interleukin-13 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight12.5kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
ConcentrationAs reported
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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