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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 15, Recombinant, Mouse (IL-15)

Interleukin 15, Recombinant, Mouse (IL-15)

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Interleukin (IL)-15 is a pleiotropic pro-inflammatory cytokine that is expressed in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. IL-15 promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. Recent emerging data suggest that IL-15 may serve as a useful therapeutic target across a range of disease states. Advances in the past year highlight the beneficial effect of IL-15 neutralisation in models of psoriasis and diabetes. Further evidence for IL-15 expression and effector function has emerged across a range of rheumatic disorders, including juvenile inflammatory arthritis, rheumatoid arthritis and Kawasaki disease. Interleukin-15 (IL-15) is a gamma-common cytokine, which plays an important role in the development, survival and proliferation of NK, NK T and CD8+ T cells.
Catalog #I8437-05A
Recombinant Murine IL-15 is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13321 Dalton.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Ile-Asp.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin 0.1ng/ug (IEU/ug) of mouse Interleukin-15.
Biological ActivityrmIL-15 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 was found to be < 5ng/ml, corresponding to a Specific Activity of 2 x 10e5 IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). .
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.
ReconstitutionReconstitute the lyophilized murine IL-15 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in 10mM Tris, pH 8.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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