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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 5, Recombinant, Rat (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Interleukin 5, Recombinant, Rat (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

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Specifications

Interleukin-5 (IL5), also known as eosinophil differentiation factor (EDF), is a lineage-specific cytokine for eosinophilpoiesis . It regulates eosinophil growth and activation, and thus plays an important role in diseases associated with increased levels of eosinophils, including asthma. IL5 has a similar overall fold to other cytokines (e.g., IL2, IL4 and GCSF), but while these exist as monomeric structures, IL5 is a homodimer. The fold contains an anti-parallel 4-alpha-helix bundle with a left handed twist, connected by a 2-stranded anti-parallel beta-sheet . The monomers are held together by 2 interchain disulfide bonds.
Catalog #I8437-06A
DescriptionRecombinant Rat IL-5 produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13074 Dalton. The Recombinant Rat IL-5 is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRat IL-5 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of BCL-1 cells was found to be < 0.15ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Recombinant Rat IL-5.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.635 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-5 as a Reference Standard.
GeneName: Il5; Synonyms: Il-5
SolubilityIt is recommended to reconstitute the lyophilized Rat IL-5 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Rat IL-5 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-5 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight13.07kD
SourceE. coli
Purity98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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