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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 15, Recombinant, Human (IL-15)

Interleukin 15, Recombinant, Human (IL-15)

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Interleukin (IL)-15 is a pleiotropic pro-inflammatory cytokine that is expressed in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. IL-15 promotes activation of T cells, neutrophils and macrophages, and is critical to dendritic cell function in several model systems. Recent emerging data suggest that IL-15 may serve as a useful therapeutic target across a range of disease states. Advances in the past year highlight the beneficial effect of IL-15 neutralisation in models of psoriasis and diabetes. Further evidence for IL-15 expression and effector function has emerged across a range of rheumatic disorders, including juvenile inflammatory arthritis, rheumatoid arthritis and Kawasaki disease. Interleukin-15 (IL-15) is a gamma-common cytokine, which plays an important role in the development, survival and proliferation of NK, NK T and CD8+ T cells.
Catalog #I8437A
Recombinant human IL-15 is a single, non-glycosylated polypeptide chain containing 114aa and a molecular mass of ~13kD. Chromatographically purified. Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val.
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 was found to be < 0.5ng/ml, corresponding to a Specific Activity of 2x10e6IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). .
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~13kD
SourceE. coli
Purity98% by RP-HPLC, SDS-PAGE.
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 5mM Tris, pH 8.0. Reconstitute with sterile ddH2O to a concentration 0.1mg.ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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