| IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation |
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| Catalog # | I8439-05N |
| Description | Recombinant Murine IL-17 produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 266 amino acids and having a molecular mass of 29,956 Dalton. Recombinant Mouse Interleukin-17 is purified by proprietary chromatographic techniques. |
| Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ala-Ile-Ile-Pro. |
| Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Biological Activity | Mouse IL-17 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent secretion of IL-6 by NIH 3T3 cells was found to be 1.0-10.0 ng/mL, |
| Endotoxin | 0.1ng/ug (IEU/ug) of mouse IL-17. |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280 nm using the absorbency value of 0.942 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard. |
| Solubility | It is recommended to reconstitute the lyophilized Recombinant Interleukin-17 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Recombinant Mouse Interleukin-17 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Mouse Interleukin-17 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Molecular Weight | 29.96kD |
| Source | E. coli |
| Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder without additives. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
