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You are here:Home » Molecular Biology » MB-Growth Factors-Interleukin » Interferon gamma, Recombinant, Human (IFN-gamma, IFNG, Immune Interferon)

Interferon gamma, Recombinant, Human (IFN-gamma, IFNG, Immune Interferon)


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Recombinant protein corresponding to 144aa of human Interferon gamma expressed in E.coli is a single, non-glycosylated, polypeptide chain.
Catalog #I8439-88
Biological ActivityThe specific activity as determined in a viral resistance assay is <0.05ng/ml, corresponding to a specific activity of 2.0x10e7 IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences(IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IFN-gamma as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~16.9kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE). Endotoxin: 0.1ng/ug
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesIFN-gamma, IFNG, Immune Interferon

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