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You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 18, Recombinant, Rat (IL-18, IFN-gamma inducing factor, IGIF)

Interleukin 18, Recombinant, Rat (IL-18, IFN-gamma inducing factor, IGIF)

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Specifications

Interleukin-18 is a member of the interleukin-1 family of cytokines with pro-inflammatory and tumor-suppressive properties. Its ability to potently enhance the production of interferon-gamma indicates in particular the crucial function of interleukin-18 as an immunomodulatory molecule. In fact, high levels of interleukin-18 are detected in human diseases associated with immunoactivation including viral or bacterial infections and chronic inflammation. Animal models suggest suppression of interleukin-18 bioactivity as a novel therapeutic concept specifically for the treatment of chronic inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis. It is the only cytokine with a unique capacity to induce T helper 1 or T helper 2 polarization, depending on the immunologic context.
Catalog #I8439-95R1
DescriptionRecombinant Rat IL-18 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. Recombinant Rat Interleukin-18 is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu.
Dimers and AggregatesLess than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRat IL-18 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IFN-gamma by murine lymph node cells (4 x 105 cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12 ng/ml. The medium was RPMI containing 10% FCS and 50µM 2-ME.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard.
Endotoxin 0.1ng/ug (IEU/ug) of rat IL-18.
SolubilityIt is recommended to reconstitute the lyophilized IL-18 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Interlekin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-18 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight18.26kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 0.4X PBS, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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