Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-Interleukin » Interleukin 20, Recombinant, Human (IL-20)

Interleukin 20, Recombinant, Human (IL-20)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

The newly discovered cytokine interleukin-20 (IL-20) is structurally related to IL-10, yet it appears to be an autocrine factor for keratinocytes that regulates their participation in inflammation.
Catalog #I8441-52
Recombinant Human IL-20 is a single, non-glycosylated homodimeric polypeptide chain containing 2x153 amino acids and having a total molecular mass of 35,212D.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityIL-20 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human MO7e cells is < 0.2ng/ml, corresponding to a Specific Activity of 5x10e6 IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of hIL-20.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of IL-20 as a Reference Standard.
ReconstitutionReconstitute the lyophilized Interleukin-20 in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRecombinant, Human from E. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in 100mM sodium chloride, 10mM sodium citrate, pH 3.0.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links